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Immunofluorescent analysis of Nanos Homologue 1 in Hela cells using a Nanos Homologue 1 polyclonal antibody (Product # PA5-35139) at a dilution of 0.025 mg/ml, followed by detection using a fluorescent conjugated secondary antibody (orange). Nuclei were stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 263-292 amino acids from the C-terminal region of human Nanos Homologue 1 (NANOS1)|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NANOS1 may regulate translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EC_Rep1a; NANOS1; NOS-1; NOS1; SPGF12; ZC2HC12A