|Tested species reactivity||Amphibian, Avian, Bovine, Chicken, Fish, Human, Mouse, Pig, Rabbit, Reptile, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified, full length, native chicken protein.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24951 detects Nebulin from chicken, mouse rat, rabbit, bovine, porcine, human, salamander, pigeon, alligator, toad, and fish samples. This antibody reacts only with skeletal muscle, and does not react with smooth muscle or non-muscle cell cultures. This antibody does not cross-react with Titin.
MA1-24951 has been successfully used in Western blot procedures.
The MA1-24951 immunogen is purified, full length, native chicken protein.
This gene encodes nebulin, a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein. The encoded protein contains approximately 30-amino acid long modules that can be classified into 7 types and other repeated modules. Protein isoform sizes vary from 600 to 800 kD due to alternative splicing that is tissue-, species-,and developmental stage-specific. Of the 183 exons in the nebulin gene, at least 43 are alternatively spliced, although exons 143 and 144 are not found in the same transcript. Of the several thousand transcript variants predicted for nebulin, the RefSeq Project has decided to create three representative RefSeq records. Mutations in this gene are associated with recessive nemaline myopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.