|Immunocytochemistry (ICC)||10 ug/ml|
|Immunofluorescence (IF)||10 ug/mL|
|Immunohistochemistry (IHC)||1 ug/ml|
|Western Blot (WB)||1 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide from near the carboxy terminus of human Nephrin.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
Despite its predicted molecular weight, Nephrin often migrates at a lower molecular weight in SDS-PAGE. A suggested positive control is mouse kidney tissue lysate.
PA5-20330 can be used with blocking peptide PEP-0450.
Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CNF, NPHN, nephrin; Nephrin; nephrin 1; nephrosis 1 homolog, nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHS1 nephrin; Renal glomerulus-specific cell adhesion receptor
Gene Aliases: CNF; nephrin; NephrinB; NPHN; NPHS1
Molecular Function: cell junction protein
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