|Tested species reactivity||Avian, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Native mouse beta NGF purified from submaxillary salivary gland.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1-3 µg/ml|
|Inhibition Assays (IA)||1-3 µg/ml|
|Immunohistochemistry (IHC)||1-3 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
By ELISA, this antibody exhibits a cross-reactivity of less than 1% to recombinant human BDNF, NT3 and NT4/5. It will not react with bovine NGF.
Use at 1-3 µg/ml for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/ml (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.
Reconstitute with 20 ul of distilled water. After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent.
Beta NGF is a nerve growth factor that is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.