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|Tested species reactivity||Mouse , Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full length native Nestin protein purified from embryonic rat spinal cord.|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA1-91657 detects Nestin from rat and mouse samples. This antibody does not cross react with human samples.
MA1-91657 has been successfully used in immunocytochemistry, immunohistochemistry (frozen tissue), Western blot, immunohistochemistry (paraffin tissue) and immunomicroscopy applications.
The MA1-91657 immunogen is: Full length native Nestin protein purified from embryonic rat spinal cord.
For use in ICC applications, cells can be fixed with 4% paraformaldehyde buffered with 50mM sodium borate at p.H. 9.5. for IHC-P and IHC-Fr tissue can be fixed with 4% paraformaldehyde at pH 7.4 for light microscopy.
The predicted molecular weight of MA1-91657 is ~200kDa.
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatocyte growth factor is a mouse fetal Leydig cell terminal differentiation factor.
MA1-91657 was used in western blot to study the role of HGF in the terminal differentiation of murine fetal Leydig cells
|Ricci G,Guglielmo MC,Caruso M,Ferranti F,Canipari R,Galdieri M,Catizone A||Biology of reproduction (87:null)||2012|
intermediate filament protein, nestin, NES, FLJ21841, Nbla00170
C78523, AA166324, ESTM46