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Immunofluorescent analysis of Nestin (green) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:100 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). Images were taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification.
|Tested species reactivity||Human , Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Human nestin protein corresponding to residues 1464-1614|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/test|
|Immunocytochemistry (ICC)||1:100 - 1:250|
|Immunofluorescence (IF)||1:20 - 1:250|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||1:500 - 1:1500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples. MA1-110 also acts a neuronal stem cell marker and detects a band in Western blot at ~250 kDa.
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Androgen receptors and gender-specific distribution of alkaline phosphatase in human thyroid cartilage.
MA1-110 was used in immunohistochemistry to study gender differences in the localization of android receptors and alkaline phosphatase in human thyroid cartilage
|Claassen H,M?nig H,Sel S,Werner JA,Paulsen F||Histochemistry and cell biology (126:381)||2006|
ESTM46, C78523, Nbla00170, AA166324
intermediate filament protein, nestin, NES, FLJ21841, Nbla00170