Immunofluorescent analysis of Nestin (green) in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). The cells were fixed and permeabilized using Image-IT® Fixation/Permeabilization kit (Product # R37602), and blocked with the including blocking buffer for one hour at room temperature. Cells were stained with a Nestin monoclonal antibody (Product # MA1-110) at a dilution of 1:100 in blocking buffer for 3 hours at room temperature, and then incubated with a DyLight488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 1 hour at room temperature. Nucleus DNA (blue) was stained with DAPI (Product # D1306). Images were taken on an EVOS® FLoid® Cell Imaging Station at 10X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human nestin protein corresponding to residues 1464-1614|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/test|
|Immunocytochemistry (ICC)||1:100 - 1:250|
|Immunofluorescence (IF)||1:20 - 1:250|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||1:500 - 1:1500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples. MA1-110 also acts a neuronal stem cell marker and detects a band in Western blot at ~250 kDa.
Nestin is expressed by many types of cells during development, although its expression is usually transient and does not persist into adulthood. One instance of nestin expression in adult organisms, and perhaps that for which nestin is best known, are the neuronal precursor cells of the subgranular zone. Nestin is an intermediate filament protein expressed in dividing cells during the early stages of development in the central nervous system, peripheral nervous system and in myogenic and other tissues. Upon differentiation, nestin becomes downregulated and is replaced by tissue-specific intermediate filament proteins. During neuro- and gliogenesis, nestin is replaced by cell type-specific intermediate filaments, e.g. neurofilaments and glial fibrillary acidic protein (GFAP). Interestingly, nestin expression is reinduced in the adult during pathological situations, such as the formation of the glial scar after CNS injury and during regeneration of injured muscle tissue. Nestin is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Embryonic stem cells markers SOX2, OCT4 and Nanog expression and their correlations with epithelial-mesenchymal transition in nasopharyngeal carcinoma.
MA1-110 was used in immunohistochemistry - paraffin section to assess the expression and prognostic impact of embryonic stem cell markers in nasopharyngeal carcinoma patients
|Luo W,Li S,Peng B,Ye Y,Deng X,Yao K||PloS one (8:null)||2013|
Androgen receptors and gender-specific distribution of alkaline phosphatase in human thyroid cartilage.
MA1-110 was used in immunohistochemistry to study gender differences in the localization of android receptors and alkaline phosphatase in human thyroid cartilage
|Claassen H,Mönig H,Sel S,Werner JA,Paulsen F||Histochemistry and cell biology (126:381)||2006|