|Tested species reactivity||Bovine, Cat, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to human Netrin 1.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is MDCKII cell lysate.
Netrin 1 and quote;s main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.