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|Tested species reactivity||Human, Rat|
|Published species reactivity||Rabbit, Fruit fly, Mouse, Human|
|Host / Isotype||Mouse|
|Immunogen||Synthetic peptide corresponding to 226 amino acids of the C-terminus of neurofilament, heavy chain.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||5 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 10 publications below|
MA1-2012 detects the neurofilament, heavy chain in human and rat samples.
MA1-2012 has been successfully used in Western blot, immunohistochemistry and immunofluorecence procedures. By Western blot this antibody detects a ~200 kDa protein representing the neurofilament, heavy chain in rat brain microsome. In immunohistochemistry procedures MA1-2012 recognizes the neurofilament, heavy chain in rat hippocampal neurons.
The MA1-2012 immunogen is a synthetic peptide corresponding to 226 amino acids of the C-terminus of neurofilament, heavy chain.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein phosphatase 5 is a major component of glucocorticoid receptor.hsp90 complexes with properties of an FK506-binding immunophilin.
MA1-2012 was used in immunoprecipitation to identify PP5 as an important component of glucocorticoid receptor-hsp90 complexes and investigate its functional properties
|Silverstein AM,Galigniana MD,Chen MS,Owens-Grillo JK,Chinkers M,Pratt WB||The Journal of biological chemistry (272:16224)||1997|
Folding of the glucocorticoid receptor by the reconstituted Hsp90-based chaperone machinery. The initial hsp90.p60.hsp70-dependent step is sufficient for creating the steroid binding conformation.
MA1-2012 was used in immunoprecipitation to study the folding of hormone binding domain of glucocorticoid receptor in vitro using reconstituted receptor-hsp90 heterocomplex assembly system with purified components
|Dittmar KD,Pratt WB||The Journal of biological chemistry (272:13047)||1997|
Functional interference between hypoxia and dioxin signal transduction pathways: competition for recruitment of the Arnt transcription factor.
MA1-2012 was used in immunoprecipitation to show HIF-1a is associated with the molecular chaperone hsp90 in vitro
|Gradin K,McGuire J,Wenger RH,Kvietikova I,fhitelaw ML,Toftgård R,Tora L,Gassmann M,Poellinger L||Molecular and cellular biology (16:5221)||1996|
A model of protein targeting mediated by immunophilins and other proteins that bind to hsp90 via tetratricopeptide repeat domains.
MA1-2012 was used in immunoprecipitation to study the mechanisms of immunophilin-mediated protein targeting
|Owens-Grillo JK,Czar MJ,Hutchison KA,Hoffmann K,Perdew GH,Pratt WB||The Journal of biological chemistry (271:13468)||1996|
|Fruit fly||Not Cited||
The basic helix-loop-helix/PAS factor Sim is associated with hsp90. Implications for regulation by interaction with partner factors.
MA1-2012 was used in immunoprecipitation to study the interaction between Sim and hsp90
|McGuire J,Coumailleau P,Whitelaw ML,Gustafsson JA,Poellinger L||The Journal of biological chemistry (270:31353)||1995|
Identification of functional domains of the aryl hydrocarbon receptor.
MA1-2012 was used in immunoprecipitation to investigate the functional domains of mouse aryl hydrocarbon receptor
|Fukunaga BN,Probst MR,Reisz-Porszasz S,Hankinson O||The Journal of biological chemistry (270:29270)||1995|
Definition of a minimal domain of the dioxin receptor that is associated with Hsp90 and maintains wild type ligand binding affinity and specificity.
MA1-2012 was used in immunoprecipitation to detect the interaction between the dioxin receptor and Hsp90
|Coumailleau P,Poellinger L,Gustafsson JA,Whitelaw ML||The Journal of biological chemistry (270:25291)||1995|
A tyrosine kinase-dependent pathway regulates ligand-dependent activation of the dioxin receptor in human keratinocytes.
MA1-2012 was used in immunoprecipitation to investigate the role of a tyrosine kinase-dependent pathway during ligand-dependent activation of the dioxin receptor in human keratinocytes
|Gradin K,Whitelaw ML,Toftgård R,Poellinger L,Berghard A||The Journal of biological chemistry (269:23800)||1994|
Characterization of the protein-protein interactions determining the heat shock protein (hsp90.hsp70.hsp56) heterocomplex.
MA1-2012 was used in immunoprecipitation to investigate the interaction of different protein components of the heat shock protein heterocomplex
|Czar MJ,Owens-Grillo JK,Dittmar KD,Hutchison KA,Zacharek AM,Leach KL,Deibel MR,Pratt WB||The Journal of biological chemistry (269:11155)||1994|
A cellular factor stimulates ligand-dependent release of hsp90 from the basic helix-loop-helix dioxin receptor.
MA1-2012 was used in immunoprecipitation to investigate the role of Arnt for release of hsp90 from the dioxin receptor in the presence of dioxin
|McGuire J,Whitelaw ML,Pongratz I,Gustafsson JA,Poellinger L||Molecular and cellular biology (14:2438)||1994|
200 kDa neurofilament protein; KIAA0845; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF-H; NFH; NHC
CMT2CC; KIAA0845; NEFH; NFH