|Tested species reactivity||Avian, Mammal|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Native pig spinal cord neurofilaments|
|Contains||5mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This antibody specifically recognizes the phosphorylated variant of NF-H subunit (~180-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
MA3-16722 was used in immunocytochemistry and western blot to study the subcellular localization of alpha internexin and neurofilament triplet proteins
|Benson DL,Mandell JW,Shaw G,Banker G||Journal of neurocytology (25:181)||1996|