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|Tested species reactivity||Bovine, Cat, Human, Mouse, Pig, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified by NF-H protein from bovine intermediate filaments prepared from spinal cords.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000-1:10,000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000-1:10,000|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
Neurofilaments can be defined as the intermediate of 10nm filaments found specifically in neuronal cells. Neurofilaments appears as fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid axon impulse conduction. They are composed of a mixture of subunits which usally include the three neurofilament triplet proteins, known as NF-L, NF-M, and NF-H. Neurofilaments may also include smaller amounts of peripherin, a-internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type marker since the proteins are among the mose abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Degeneration and regeneration of corneal nerves in response to HSV-1 infection.
PA3-16753 was used in immunohistochemistry - frozen section to characterize corneal nerve network alterations induced by HSV-1 infection
|Chucair-Elliott AJ,Zheng M,Carr DJ||Investigative ophthalmology & visual science (56:1097)||2015|
200 kDa neurofilament protein; KIAA0845; neurofilament 200kDa; neurofilament heavy polypeptide; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF-H; NFH; NHC
CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH