|Tested species reactivity||Bovine, Dog, Chicken, Guinea pig, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat, Xenopus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytoskeletal preparation of calf brain tissue.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OMA1-06115 specifically detects the phosphorylated 200 kDa neurofilament from Human, Bovine, Canine, Chicken, Guinea Pig, Non-human primate, mouse, Rabbit, Rat, Ovine and Xenopus tissues.
OMA1-06115 has been successfully used in Western blot, IHC (F), and IHC (P) procedures. By Western blot, this antibody detects an ~200 kDa protein representing the phosphorylated form of the 200 kDa neurofilament from human brain homogenate.
OMA1-06115 antigen is cytoskeletal preparation of calf brain tissue.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.