|Tested species reactivity||Bovine, Dog, Chicken, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Rat, Xenopus|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cytoskeletal preparation of calf brain tissue.|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50 - 1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100 - 1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OMA1-06117 reacts exclusively with the phosphorylated form of the 200 kDa neurofilament from human, mouse, rat, canine, rabbit, monkey, bovine, ovine, hamster, chicken, guinea pig, and Xenopus laevis tissues.
OMA1-06117 has been successfully used in Western blot and IHC (P) procedures. By Western blot, this antibody detects a single ~200 kDa protein representing phosphorylated neurofilament from rat brain homogenate.
OMA1-06117 antigen is cytoskeletal preparation of calf brain tissue.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.