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|Tested species reactivity||Bovine, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified pig full-length NF-L|
|Storage buffer||whole serum|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western blot using alkaline phosphatase plus chromagen, a dilution of at least 1:1000 is recommended. For chemiluminescence, use at a dilution of 1:20,000.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
68 kDa neurofilament protein; 70 kDa neurofilament protein; CMT1F; CMT2E; light molecular weight neurofilament protein; micro glutamic acid-rich protein; neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet L pro; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF-L; NFL; NLC; protein phosphatase 1, regulatory subunit 110
AI847934; BOS_9100; CMT1F; CMT2E; NEFL; NF-L; NF68; NFL; PPP1R110