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Immunofluorescent analysis of the neurofilament medium chain in paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). Tissue sections were deparaffinized with xylene, and rehydrated with ethanol. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0) and microwaved for 8-15 min. Following antigen retrieval, tissues were washed with water and PBS, and then blocked in 0.3% BSA for 30 min at room temperature. Tissues were then probed with a neurofilament medium chain monoclonal antibody (Product # 13-0500) in 0.3% BSA at a dilution of 1:50 for 1 hour at 37°C. Tissues were then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate for 1 hour at 37°C (green). Nuclei (blue) were stained with DAPI. Images were taken at 40X magnification.
|Tested species reactivity||Human, Leech, Rat|
|Published species reactivity||Zebrafish, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5-10 ug/ml|
|Immunoprecipitation (IP)||2-5 ug|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-M is the medium molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 160 kDa.
Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some variation in different species. NF-H polyclonal antibody can be used to identify neurons and their processes in tissue sections and in tissue culture and the antibody can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Rapid reverse genetic screening using CRISPR in zebrafish.
13-0500 was used in immunohistochemistry to identify novel genes involved in electrical-synapse formation using CRISPR in zebrafish.
|Shah AN,Davey CF,Whitebirch AC,Miller AC,Moens CB||Nature methods (12:535)||2015|
Unique function of Kinesin Kif5A in localization of mitochondria in axons.
13-0500 was used in immunohistochemistry to characterize neuronal defects in zebrafish kif5Aa mutants
|Campbell PD,Shen K,Sapio MR,Glenn TD,Talbot WS,Marlow FL||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:14717)||2014|
|Zebrafish||Not Cited||The zebrafish Iroquois gene iro7 positions the r4/r5 boundary and controls neurogenesis in the rostral hindbrain.||Lecaudey V,Anselme I,Rosa F,Schneider-Maunoury S||Development (Cambridge, England) (131:3121)||2004|
vhnf1 and Fgf signals synergize to specify rhombomere identity in the zebrafish hindbrain.
13-0500 was used in immunohistochemistry to elucidate the mechanism of rhombomere determination.
|Wiellette EL,Sive H||Development (Cambridge, England) (130:3821)||2003|
|Mouse||1:1000||Abnormal neurofilament transport caused by targeted disruption of neuronal kinesin heavy chain KIF5A.||Xia CH,Roberts EA,Her LS,Liu X,Williams DS,Cleveland DW,Goldstein LS||The Journal of cell biology (161:55)||2003|
|Zebrafish||1:25||FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain.||Maves L,Jackman W,Kimmel CB||Development (Cambridge, England) (129:3825)||2002|
160 kDa neurofilament protein; medium polypeptide 150kDa; NEF3; neurofilament; neurofilament 3; neurofilament 3, medium; neurofilament medium polypeptide; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NFM
NEF3; NEFM; NF-M; NFM