Western blot of whole rat cerebellum homogenate stained with PA3-16720, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~145kDa corresponds to rodent NF-M. Human, cow and bovine NF-M run a little slower, at about 160kDa.
|Tested species reactivity||Avian, Bovine, Cat, Human, Mouse, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant C-terminal segment of rat NF-M|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000-1:5000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000-1:5000|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is likely to react with most mammals.
Suggested positive control: antigen standard for NEFM (transient overexpression lysate).
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.