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|Tested species reactivity||Hamster, Human, Non-human primate, Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytoskeletal preparation of calf brain tissue.|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
OMA1-06110 detects phosphorylated 160 kDa neurofilament from human, hamster, non-human primate and xenopus tissues.
OMA1-06110 has been successfully used in Western blot and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects an ~160 kDa protein representing the phosphorylated form of the 160 kDa neurofilament from human nervous tissue extract.
OMA1-06110 antigen is cytoskeletal preparation of calf brain tissue.
Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. The neurofilament (NF) triplet proteins (~70, 160, and 200 kDa) occur in both the central and
peripheral nervous system and are normally restricted to neurons. The 70 kDa NF-protein can self-assemble into a filamentous structure, whereas the 160 kDa and 200 kDa NF-proteins require the presence of the 70 kDa NF-protein to co-assemble.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
160 kD neurofilament protein; 160 kDa neurofilament protein; NEF3; Neurofilament 3; neurofilament 3, medium; Neurofilament medium polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NF-M; NFM; NMC
nef-m; NEF3; NEFM; NF-M; NFM