Immunofluorescence analysis of Neurokinin 1 Receptor / NK1R Receptor was performed using 90% confluent log phase Neuro 2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Neurokinin 1 Receptor / NK1R (ZN003) Mouse Monoclonal Antibody (Product # 39-6100) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Insect, Mouse, Rat|
|Published species reactivity||Mammal|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from an internal sequence of the human, mouse, and rat NK1 receptor proteins|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Tachykinin Receptor 1, a member of the Tachykinin Receptor subfamily, is involved in the mediation of phosphatidylinositol metabolism by the tachykinin substance P, also referred to as neurokinin 1. It is associated with the management of depression, anxiety, and stress. It is also involved in regulating behavioral responses, neuronal survival, and cardiovascular and respiratory functions. The tachykinin receptor 1 is implicated in the progression of rheumatoid arthritis and osteoarthritis. Two variants of the tachykinin receptor 1 are produced by alternative splicing. Tachykinin receptor 1 has been reported primarily in brain, and in blood lymphocytes, nose, small intestine, and stomach. ESTs have been isolated from brain and spleen libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclophosphamide causes activation of protein kinase A (PKA) in the brainstem of vomiting least shrews (Cryptotis parva).
39-6100 was used in western blot to study the activation of brain stem PKA in vomiting least shrews treated with the emetic compound cyclophosphamide.
|Alkam T,Chebolu S,Darmani NA||European journal of pharmacology (722:156)||2014|