Invitrogen

Neuroligin 2 (extracellular) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (7) Neuroligin 2 antibodies

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Questions & Answers

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Neuroligin 2 (extracellular) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Neuroligin 2 (extracellular) in rat PC-12 cells. A) Samples were probed with Neuroligin 2 (extracellular) polyclonal antibody (Product # PA5-77523) using a dilution of 1:25, and incubated with goat anti-rabbit-AlexaFluor-594. B) Nuclei stained image. C) Merged image of Panels A and B.

Neuroligin 2 (extracellular) Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of Neuroligin 2 (extracellular) in rat reticular thalamic nucleus. A) Samples were probed with Neuroligin 2 (extracellular) polyclonal antibody (Product # PA5-77523) and incubated with DAPI. B) Nuclei stained image. C) Merged image of Panels A and B.

Neuroligin 2 (extracellular) Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of Neuroligin 2 (extracellular) in rat reticular thalamic nucleus. A) Samples were probed with Neuroligin 2 (extracellular) polyclonal antibody (Product # PA5-77523) and incubated with DAPI. B) Nuclei stained image. C) Merged image of Panels A and B.

Neuroligin 2 (extracellular) Antibody in Western Blot (WB) — Western blot was performed using Anti-Neuroligin 2 (extracellular) Polyclonal Antibody (Product # PA5-77523) and a 90 kDa band corresponding to NLGN2 was observed across Mouse brain, Mouse cerebellum and Rat brain but not in Mouse eye, Mouse Liver and Mouse Skeletal muscle, which are known to be tissues that do not express NLGN2. Tissue extracts (30 µg lysate) of Mouse brain (Lane 1), Mouse cerebellum (Lane 2), Mouse eye (Lane 3), Mouse liver (Lane 4), Mouse skeletal muscle (Lane 5), and Rat brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Neuroligin 2 (extracellular) Antibody in Western Blot (WB) — Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and rat PC-12 cells (lanes 3 and 6) with Neuroligin 2 (extracellular) polyclonal antibody (Product # PA5-77523) using a dilution of 1:400.

Neuroligin 2 (extracellular) Antibody — (C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat Neuroligin 2

Neuroligin 2 (extracellular) Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues tested owing to their inherent genetic constitution. Relative expression of NLGN2 was observed in Mouse brain, Mouse cerebellum and Rat brain as compared to Mouse eye, Mouse liver and Mouse skeletal muscle using Neuroligin 2 (extracellular) Polyclonal Antibody (Product # PA5-77523) in western blot analysis. {RE}

Product Details

PA5-77523

Applications	Tested Dilution	Publications
Western Blot (WB)	1:400	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1:25	-

Product Specifications
Species Reactivity	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide (C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat Neuroligin 2 View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.85 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2736214

Product Specific Information

Product is shipped at room temperature as a lyophilized powder and should be stored at -20 °C upon receipt. Reconstitution: add 50 µL of deionized water.

Target Information

This gene encodes a member of a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Neuroligin-2; ortholog of human and rat neuroligin 2 NLGN2

Gene Aliases: Kiaa1366; NL2; Nlgn2

UniProt ID: (Rat) Q62888, (Mouse) Q69ZK9

Entrez Gene ID: (Rat) 117096, (Mouse) 216856

Molecular Function: cell adhesion molecule

thigmotaxis regulation of respiratory gaseous exchange by neurological system process cell adhesion neuron cell-cell adhesion synapse assembly jump response metabolic process sensory perception of pain positive regulation of synaptic transmission, GABAergic social behavior protein localization to synapse locomotory exploration behavior modulation of synaptic transmission synapse organization neuromuscular process controlling balance positive regulation of synapse assembly positive regulation of synaptic transmission, glutamatergic terminal button organization postsynaptic membrane assembly presynaptic membrane assembly gephyrin clustering involved in postsynaptic density assembly postsynaptic density protein 95 clustering positive regulation of inhibitory postsynaptic potential regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential positive regulation of cell proliferation positive regulation of insulin secretion positive regulation of dendritic spine development insulin metabolic process positive regulation of protein localization to synapse positive regulation of t-SNARE clustering positive regulation of synaptic vesicle clustering

Process(es)

receptor activity neurexin family protein binding cell adhesion molecule binding carboxylic ester hydrolase activity protein binding