Invitrogen

Neuromedin U-8 Polyclonal Antibody

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Neuromedin U-8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Neuromedin U-8 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Neuromedin U-8 polyclonal antibody (Product # PA5-101715) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Neuromedin U-8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Neuromedin U-8 in human Melanoma tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Neuromedin U-8 polyclonal antibody (Product # PA5-101715) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Neuromedin U-8 Antibody in Western Blot (WB) — Western blot analysis of Neuromedin U-8 in HepG2 cells. Samples were incubated with Neuromedin U-8 polyclonal antibody (Product # PA5-101715).

Product Details

PA5-101715

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human NMU, corresponding to a region within C-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2851148

Target Information

Neuromedin U (NmU) is a vertebrate peptide which stimulates uterine smooth muscle contraction and causes selective vasoconstriction. Like most other active peptides, it is proteolytically processed from a larger precursor protein. The mature peptides are 8 (NmU-8) to 25 (NmU-25) residues long and C-terminally amidated. The sequence of the C-terminal extremity of NmU is extremely well conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Neuromedin precursor-related peptide 33; Neuromedin precursor-related peptide 36; Neuromedin-U; Neuromedin-U-25; NmU 25; NmU-25; NURP33; NURP36; prepro-NMU

Gene Aliases: NMU

UniProt ID: (Human) P48645

Entrez Gene ID: (Human) 10874

Function(s)