Invitrogen

Neuromedin U-8 Polyclonal Antibody

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Neuromedin U-8 Antibody in Western Blot (WB) — Western blot analysis of Neuromedin U-8 in HepG2 cells. Samples were incubated with Neuromedin U-8 polyclonal antibody (Product # PA5-101715).

Neuromedin U-8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Neuromedin U-8 in human Melanoma tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Neuromedin U-8 polyclonal antibody (Product # PA5-101715) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Neuromedin U-8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Neuromedin U-8 in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Neuromedin U-8 polyclonal antibody (Product # PA5-101715) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NMU, corresponding to a region within C-terminal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2851148

Target Information

Neuromedin U (NmU) is a vertebrate peptide which stimulates uterine smooth muscle contraction and causes selective vasoconstriction. Like most other active peptides, it is proteolytically processed from a larger precursor protein. The mature peptides are 8 (NmU-8) to 25 (NmU-25) residues long and C-terminally amidated. The sequence of the C-terminal extremity of NmU is extremely well conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Neuromedin precursor-related peptide 33; Neuromedin precursor-related peptide 36; Neuromedin-U; Neuromedin-U-25; NmU 25; NmU-25; NURP33; NURP36; prepro-NMU

Gene Aliases: NMU

UniProt ID: (Human) P48645

Entrez Gene ID: (Human) 10874

Function(s)