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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant, full length, human NSE protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:300|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20075 detects NSE in human samples.
MA1-20075 has been successfully used in ELISA, Immunohistochemistry (paraffin) and Western blot procedures. Detects a band ~45 kDa.
The MA1-20075 immunogen is recombinant, full length, human NSE protein.
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Eno; ENOG; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; glycerate hydrolyase; Neural Enolase; Neuron Specific Enolase; neuron specific gamma enolase; neuron-specific enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase
ENO2; HEL-S-279; NSE