|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified Neuron Specific Enolase from human brain|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:300|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:300|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-22730 detects NSE in Human samples.
MA1-22730 has been successfully used in ELISA, Immunohistochemistry (Frozen) and Immunohistochemistry (Paraffin) procedures.
MA1-22730 immunogen corresponds to Purified Neuron Specific Enolase from human brain.
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.