Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Dog|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full length human NSE purified from E. coli.|
|Storage buffer||whole serum|
|Contains||10mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Suggested positive control: antigen standard for ENO2 (transient overexpression lysate), rat spinal cord or peripheral nerve homogenate and HEK 293 lysate..
Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers. NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. Perhaps not surprisingly since neurons require a great deal of energy they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Valproic acid, a histone deacetylase inhibitor, decreases proliferation of and induces specific neurogenic differentiation of canine adipose tissue-derived stem cells.
PA1-46203 was used in immunocytochemistry to investigate the effects of valproic acid administration on canine adipose tissue-derived stem cells
|Kurihara Y,Suzuki T,Sakaue M,Murayama O,Miyazaki Y,Onuki A,Aoki T,Saito M,Fujii Y,Hisasue M,Tanaka K,Takizawa T||The Journal of veterinary medical science (76:15)||2014|