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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human NSE.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:300|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is pancreas tissue.
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2-phospho-glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimmers composed of three distinct subunits (α, β and γ). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolyase; Enolase 2; enolase 2 (gamma, neuronal); epididymis secretory protein Li 279; Gamma-enolase; Neural enolase; neuron specific gamma enolase; Neuron-specific enolase; neuronal enriched enolase; neurone-specific enolase; NSE
ENO2; HEL-S-279; NSE