|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: D(161) G K L T L Q E F Q E G S K A D P S I V Q A L S L Y D G L V(190)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-939 detects neuronal calcium sensor-1 (NCS-1) from rat tissues.
PA1-939 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~20 kDa protein representing NCS-1 from rat brain extract.
PA1-939 immunizing peptide corresponds to amino acid residues 161-190 from rat NCS-1. This sequence is completely conserved in mouse, rat, chicken and human NCS-1. There is a single amino acid substitution in this region for Xenopus NCS-1.
The neuronal calcium sensor (NCS) proteins are bind calcium through EF-hand motifs and are predominantly expressed in neurons. Photoreceptor cells express type A sensors and include the proteins recoverin, S-modulin, and visinin. Type B receptors are expressed in neurons and include NCS1 and the Drosophila homolog frequenin, and VILIP. NCS1 has been shown to regulate neurosecretion in a calcium-dependent manner, potentiates nitric oxide synthase activity, and can activate calcineurin and 3'-5' nucleotide phosphodiesterase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.