ELISA plates (96 well) were coated with various peptides (Columns 1-7) and BSA (Column 8) at 0.5 µg/well. Samples were incubated with a Neuropeptide Y polyclonal antibody (Product # PA1-24891) at a dilution of 1:5000 followed by detection using a goat anti-rabbit IgG-Alkaline Phosphatase secondary antibody at a dilution of 1:5000 with a PNPP substrate; Columns (left to right) 1. Neuropeptide Y (NPY) (sheep) 2. Peptide YY (PYY) (human) 3. Neuropeptide Y (NPY) (porcine) 4. Neuropeptide Y (NPY) (human) 5. Neurokinin B (NKB) (human) 6. Neurokinin A (NKA) (human) 7. Substance P (SP) 8. Albumin, bovine (BSA).
|Tested species reactivity||Bovine, Cat, Human, Mouse, Non-human primate, Pig, Rabbit, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Neuropeptide Y, conjugated to KLH, corresponding to the porcine protein.|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:8,000|
|Radioimmune Assays (RIA)||1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24891 detects Neuropeptide Y from human, mouse, rat, feline, bovine, monkey, porcine, and rabbit samples. PA1-24891 is expected to cross react with chicken (99% conserved) due to sequence homology.
PA1-24891 has been successfully used in radioimmune assay procedures.
The PA1-24891 immunogen is a synthetic Neuropeptide Y, conjugated to KLH, corresponding to the porcine protein.
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.