Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human cerebral cortex tissue using PA5-19568, anti-Neuropeptide Y antibody. Primary antibody was used at a concentration of 1 ug/ml and exposed for 8 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6 / 20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
|Tested species reactivity||Human, Mouse, Non-human primate, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Pig Neuropeptide Y.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:3000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with sheep, rabbit, chicken, guinea pig, cow, pigeon, pig, zebrafish and rhesus monkey based on sequence homology.
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase , regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.