Western blot analysis was performed on tissue extracts (30 ug lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2). The blot was probed with Neuropilin 1 Rabbit polyclonal Antibody (Product# 34-7300, 2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 025 ug/ml, 1:4000 dilution). A 68 kDa band corresponding to Neuropilin 1 was observed across the tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal sequence of the human Neuropilin-1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Neuropilin 1 is a 923 amino acid containing membrane bound coreceptor to a tyrosine kinase receptor belonging to the neuropilin family with two CUB domains, two F5/8 type C domains and a MAM domain. Neuropilin 1 forms a heterodimer with NRP2 (Neuropilin 2) and is known to bind to plexin (PLXNB1) to form semaphorin receptors. Expressed mostly in the nervous system this receptor is implicated in a wide range of functions including in the development of the cardiovascular system, angiogenesis, repulsive axon guidance, formation of certain neuronal circuits, cell survival, control of neuronal migration and organogenesis outside the nervous system. This semaphorin receptor functions as receptors for both the semaphorins and vascular endothelial growth factor and is also a regulator of VEGF-induced angiogenesis. Neuropilin 1 may be involved in aggressive advanced prostate carcinoma and in the progression of breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Prognostic implication of neuropilin-1 upregulation in human nasopharyngeal carcinoma.
34-7300 was used in immunohistochemistry (paraffin) to assess the role of neuropilin-1 in human nasopharyngeal carcinoma.
|Xu Y,Li P,Zhang X,Wang J,Gu D,Wang Y||Diagnostic pathology (8:null)||2013|
Downregulation of neuropilin-1 in patients with acute myeloid leukemia treated with thalidomide.
34-7300 was used in immunohistochemistry - paraffin section to investigate NRP-1 in refractory or relapsed acute myeloid leukemia patients
|Kreuter M,Steins M,Woelke K,Buechner T,Berdel WE,Mesters RM||European journal of haematology (79:392)||2007|
Correlation of neuropilin-1 overexpression to survival in acute myeloid leukemia.
34-7300 was used in immunohistochemistry - paraffin section to examine the contribution of neuropilin-1 to acute myeloid leukemia
|Kreuter M,Woelke K,Bieker R,Schliemann C,Steins M,Buechner T,Berdel WE,Mesters RM||Leukemia (20:1950)||2006|
|Vascular endothelial growth factor induces branching morphogenesis/tubulogenesis in renal epithelial cells in a neuropilin-dependent fashion.||Karihaloo A,Karumanchi SA,Cantley WL,Venkatesha S,Cantley LG,Kale S||Molecular and cellular biology (25:7441)||2005|