|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Purified BALB/c mouse neutrophils.|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Functional Assay (FN)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40038 detects Neutrophils from mouse samples.
MA1-40038 has been successfully used in flow cytometry, immunohistochemistry (frozen), Immuno Assays, functional assay, and immunohistochemistry (paraffin) applications. It is recommended that users test the reagent and determine their own optimal starting dilutions. The typical starting working dilution is 1:50. Suggested positive control of mouse neutrophils. Suggested negative control of mouse thymocytes.
The immunogen used to produce MA1-40038 is purified BALB/c mouse neutrophils.
The monoclonal antibody NIMP-R14 has a high specificity for murine Ly-6G and Ly-6C. The Ly-6G/C locus encodes far a family of Ly-6 proteins including Ly-6G and Ly-6C. These proteins are expressed on murine neutrophils, a subset of eosinophils, a differentiating pre-monocytes and plasmacytoid dendritic cells. The monoclonal antibody NIMP-R14 has been succesfully used to stain polymorphonnuclear (PMN) cells and monocytes for fluorecent activated cell sorting and in frozen and parffin sections. Furthermore it has been shown to be useful for depletion of neutrophils in mices. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||1 µg/ml||
Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair.
MA1-40038 was used in immunohistochemistry - paraffin section to investigate promotion of healing in a bacterial LPS model of delayed cutaneous wound repair by oestrogen
|Crompton R,Williams H,Ansell D,Campbell L,Holden K,Cruickshank S,Hardman MJ||Laboratory investigation; a journal of technical methods and pathology (96:439)||2016|
Cholesterol-Independent Suppression of Lymphocyte Activation, Autoimmunity, and Glomerulonephritis by Apolipoprotein A-I in Normocholesterolemic Lupus-Prone Mice.
MA1-40038 was used in immunohistochemistry - frozen section to study apolipoprotein-1 during normocholesterolemic conditions
|Black LL,Srivastava R,Schoeb TR,Moore RD,Barnes S,Kabarowski JH||Journal of immunology (Baltimore, Md. : 1950) (195:4685)||2015|