Western blot analysis of Nicotinic Acetylcholine Receptor beta 2 using 50µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Nicotinic Acetylcholine Receptor beta 2 polyclonal antibody (Product # PA5-29792) at a dilution of 1:5000.
|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 25 and 273 of Human Nicotinic Acetylcholine Receptor beta 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29792 targets Nicotinic Acetylcholine Receptor beta 2 in WB applications and shows reactivity with mouse samples.
The PA5-29792 immunogen is recombinant fragment corresponding to a region within amino acids 25 and 273 of Human Nicotinic Acetylcholine Receptor beta 2.
Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy.
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