|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide near the C-terminus of mouse iNOS protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
Inducible NOS (iNOS) or macrophage NOS (mNOS) is calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. NOS oxidizes a guanidine nitrogen of arginine, releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation, neurotransmission, anti-tumor and anti-pathogenic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression and prognostic significance of iNOS in uveal melanoma.
MA5-16422 was used in immunohistochemistry to study the prognostic value of iNOS expression in uveal melanoma
|Johansson CC,Mougiakakos D,Trocme E,All-Ericsson C,Economou MA,Larsson O,Seregard S,Kiessling R||International journal of cancer (126:2682)||2010|