|Tested species reactivity||Chemical|
|Published species reactivity||Rat, Pig, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Nitrotyrosine attached to a carrier protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1-2 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Nitrotyrosine is a relatively stable product formed from various reaction pathways. Perhaps most notable is the reaction of peroxynitrite (formed from Superoxide and nitric oxide radicals) with tyrosine. As a strong oxidant and nitrating agent, peroxynitrite mediates tyrosine nitration reactions on proteins resulting in inactivation of certain housekeeping enzymes (e. g. alpha1-antiproteinase) as well as endogenous antioxidant enzymes such as catalase and SOD. Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Nitrotyrosine has been implicated in the pathogenesis of several inflammatory, infectious and degenerative human diseases, such as Alzheimer's disease, amyotrophic lateral sclerosis (ALS), asthma, atherosclerosis and a variety of conditions precipitated by endothelial injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Peroxynitrous acid induces structural and functional modifications to basement membranes and its key component, laminin.
32-1900 was used in western blot to report the co-localization of 3-nitrotyrosine and laminin in human atherosclerotic lesions.
|Degendorfer G,Chuang CY,Hammer A,Malle E,Davies MJ||Free radical biology and medicine (89:721)||2015|
Oxidized LDL in human carotid plaques is related to symptomatic carotid disease and lesion instability.
32-1900 was used in immunohistochemistry - paraffin section to study the role of oxidative stress in plaque erosion and symptomatic carotid disease
|Sigala F,Kotsinas A,Savari P,Filis K,Markantonis S,Iliodromitis EK,Gorgoulis VG,Andreadou I||Journal of vascular surgery (52:704)||2010|
|Not Applicable||Not Cited||
Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding.
32-1900 was used in western blot to investigate nitration of PECAM-1.
|Newman DK,Hoffman S,Kotamraju S,Zhao T,Wakim B,Kalyanaraman B,Newman PJ||Biochemical and biophysical research communications (296:1171)||2002|
Evaluation of a method for nitrotyrosine site identification and relative quantitation using a stable isotope-labeled nitrated spike-in standard and high resolution fourier transform MS and MS/MS analysis.
32-1900 was used in western blot to evaluation an approach for nitrotyrosine site identification and relative quantitation.
|Seeley KW,Fertig AR,Dufresne CP,Pinho JP,Stevens SM||International journal of molecular sciences (15:6265)||2014|
|Not Applicable||Not Cited||
Nitric oxide synthase mediates PC12 differentiation induced by the surface topography of nanostructured TiO2.
32-1900 was used in western blot to investigate the molecular mechanism through which cells sense and adapt to the substrate.
|Tamplenizza M,Lenardi C,Maffioli E,Nonnis S,Negri A,Forti S,Sogne E,De Astis S,Matteoli M,Schulte C,Milani P,Tedeschi G||Journal of nanobiotechnology (11:null)||2013|
|Rat||Not Cited||Role for complement in mediating intestinal nitric oxide synthase-2 and superoxide dismutase expression.||Montalto MC,Hart ML,Jordan JE,Wada K,Stahl GL||American journal of physiology. Gastrointestinal and liver physiology (285:G197)||2003|
|Mouse||Not Cited||Histone H1.2 is a substrate for denitrase, an activity that reduces nitrotyrosine immunoreactivity in proteins.||Irie Y,Saeki M,Kamisaki Y,Martin E,Murad F||Proceedings of the National Academy of Sciences of the United States of America (100:5634)||2003|
Chronic intermittent hypoxia induces local inflammation of the rat carotid body via functional upregulation of proinflammatory cytokine pathways.
32-1900 was used in immunohistochemistry - paraffin section to study intermittent hypoxia-induced inflammation in the carotid body.
|Lam SY,Liu Y,Ng KM,Lau CF,Liong EC,Tipoe GL,Fung ML||Histochemistry and cell biology (137:303)||2012|
Inducible nitric oxide synthase, nitrotyrosine and p53 mutations in the molecular pathogenesis of Barrett's esophagus and esophageal adenocarcinoma.
32-1900 was used in immunohistochemistry - paraffin section to determine the contributions of nitric oxide in esophageal adenocarcinoma
|Vaninetti NM,Geldenhuys L,Porter GA,Risch H,Hainaut P,Guernsey DL,Casson AG||Molecular carcinogenesis (47:275)||2008|
|Not Applicable||Not Cited||
Ginseng, the root of Panax ginseng C.A. Meyer, protects ethanol-induced gastric damages in rat through the induction of cytoprotective heat-shock protein 27.
32-1900 was used in immunohistochemistry - paraffin section to analyze the protective effects of ginseng against ethanol-induced gastric damages in rat
|Yeo M,Kim DK,Cho SW,Hong HD||Digestive diseases and sciences (53:606)||2008|
Effects of plant alkylphenols on cytokine production, tyrosine nitration and inflammatory damage in the efferent phase of contact hypersensitivity.
32-1900 was used in immunohistochemistry - paraffin section to link the anti-inflammatory effect of phenolic compounds isoprenylhydroquinone glucoside and 3,5-dicaffeoylquinic acid to cytokine release and tyrosine nitration in skin
|Olmos A,Giner RM,Recio MC,Rios JL,Cerdá-Nicolás JM,Máñez S||British journal of pharmacology (152:366)||2007|
|Mouse||Not Cited||Protective effect of endogenous PPARgamma against acute gastric mucosal lesions associated with ischemia-reperfusion.||Wada K,Nakajima A,Takahashi H,Yoneda M,Fujisawa N,Ohsawa E,Kadowaki T,Kubota N,Terauchi Y,Matsuhashi N,Saubermann LJ,Nakajima N,Blumberg RS||American journal of physiology. Gastrointestinal and liver physiology (287:G452)||2004|
||Role for complement in mediating intestinal nitric oxide synthase-2 and superoxide dismutase expression.||Montalto MC,Hart ML,Jordan JE,Wada K,Stahl GL||American journal of physiology. Gastrointestinal and liver physiology (285:G197)||2003|
|Pig||1:100||Evidence of nitric oxide synthase 2 activity in swine naturally infected with Actinobacillus pleuropneumoniae.||Cho WS,Chae C||Veterinary pathology (40:276)||2003|