Immunofluorescent analysis of Nkx2.2 showing staining in the nucleus of SKNSH cells. SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Nkx2.2 polyclonal antibody (Product # PA5-30862) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 60 and 273 of Human Nkx2.2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30862 targets Nkx2.2 in WB applications and shows reactivity with Human samples.
The PA5-30862 immunogen is recombinant fragment corresponding to a region within amino acids 60 and 273 of Human Nkx2.2.
The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.
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