Immunocytochemistry analysis of Human cardiomyocytes stained with ABfinity™ Nkx2.5 Recombinant Rabbit Oligoclonal antibody (5.0 µg/mL) using a: Alexa Fluor® 488 goat anti-rabbit as a secondary antibody (green). b: DAPI stained Primary human cardiomyocytes nuclei (blue). c: Actin stained with Alexa Fluor® 594 Phalloidin (red). d: composite image of cells showing nuclear localization. e: No primary control.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NKX2-5 is a transcription factor protein which plays a crucial role in cardiac development and differentiation. It is a low molecular weight protein of 35-38 kDa depending on phosphorylation status. Expression is mainly restricted to the heart. But during embryogenesis NKX2-5 expression has also been detected in spleen-precursor cells. Over expression of NKX2-5 resulted in expression of neuronal markers in C2C12 murine myogenic cells. Functionally it is involved in differentiation processes in heart development and in homeostasis and survival of cardiac myocytes as well maintains the initial muscle differentiation paradigm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.