Immunofluorescent analysis of Nociceptin using a Nociceptin polyclonal antibody (Product # PA1-27972). Confocal images showing OFQ (red), GnRH (green) and their colocalization (yellow) in the ovine preoptic area and hypothalamus. A-C: Low power view of the preoptic area at the level of the organum vasculosum of the lamina terminalis (OVLT) showing OFQ and GnRH cells, and their colocalization (e.g., white arrows). Bar = 100µm D-F: High power images of a double-labeled OFQ/GnRH cell; note that the immunofluorescence for OFQ has a punctateappearance in the cytoplasm as opposed to the more diffuse appearance of the GnRH signal. Bar 26 = 20µm G-I: Single-labeled OFQ cells (e.g., red arrow) in the arcuate nucleus. Note that many GnRH fibers coursing through this region are also OFQ-positive (e.g., white arrows). 3v = third ventricle. Bar = 100µm.
|Tested species reactivity||Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide FGGFTGARKSARKLANQ, corresponding to amino acids 122-138 of Rat Orphanin FQ.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27972 detects Nociceptin from rat, non-human primate samples.
PA1-27972 has been successfully used in immunohistochemistry (frozen tissue) applications.
The PA1-27972 immunogen is: Synthetic peptide: FGGFTGARKSARKLANQ, corresponding to amino acids 122-138 of Rat Orphanin FQ.
Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.