Western blot analysis of Nonphospho-4E-BP1 (Thr46) in extracts from COS cells, treated with lambda phosphatase or 20% FBS, using Nonphospho-4E-BP1 (Thr46) monoclonal antibody (Product # MA5-15032) (upper), Phospho-4E-BP1 (Thr37/46) antibody (middle), and 4E-BP1 antibody (lower).
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding pThr46 of human 4E-BP1|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.