Extracts of human A431 cells untreated (co) or treated with EGF (EGF) (approximately 20,000 cells per lane), were resolved by SDS-PAGE and transferred to PVDF. The membrane was blocked with a casein/Tween 20 buffer then incubated with mouse anti-EGFR [npY1173] antibody (Catalog no. 44794M) at 0.5 µg/mL for 1 hour at room temperature. After washing, the membrane was incubated with an anti-mouse HRP-conjugated secondary antibody and signals were detected using an ECL detection method (exposure time: 30 seconds).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide derived from the region of EGFR containing tyrosine 1173 (dephosphorylated), conjugated to KLH.|
|Storage buffer||PBS, pH 7.3, with PEG, sucrose|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
Epidermal Growth Factor Receptor (EGFR) is a 175 kDa transmembrane glycoprotein belonging to tyrosine kinase superfamily. It acts as a receptor for epidermal growth factor (EGF) family proteins. Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction pathways connected to cell proliferation and differentiation. Tyrosine 1068 present within the cytoplasmic domain of the receptor is a major autophosphorylation site that allows binding of Grb2 and activation of the Ras-Raf-ERK1 and ERK2 signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Molecular classification of head and neck squamous cell carcinomas using patterns of gene expression.
44794M was used in immunohistochemistry - paraffin section to identify biomarkers for head and neck squamous cell carcinoma
|Chung CH,Parker JS,Karaca G,Wu J,Funkhouser WK,Moore D,Butterfoss D,Xiang D,Zanation A,Yin X,Shockley WW,Weissler MC,Dressler LG,Shores CG,Yarbrough WG,Perou CM||Cancer cell (5:489)||2004|