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|Tested species reactivity||Many|
|Published species reactivity||Pig, Hamster, Fish, Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||Purified NCC cells from catfish.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||Assay dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-760 detects nonspecific cytotoxic cells (NCC) from catfish and tilapia tissues and also detects natural killer/lymphokine activated killer (NK/LAK) cells from rat, bovine, equine, canine and porcine tissues. MA1-760 has been successfully used in Western blot, cytotoxic inhibition studies with NCC/NK/LAK cells, FACS, immunoprecipitation, panning, and ELISA procedures. By Western blot, this antibody detects a 32 kDa protein representing nonspecific cytotoxic cell receptor protein. In cytotoxicity assays, this antibody is used under saturating conditions to inhibit target cell conjugation. The MA1-760 immunogen is purified NCC cells from catfish.
Non-specific cytotoxic cells (NCCs) in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. One such property is that NCC/natural killer (NK)/lymphokine activated killer (LAK) cells express spontaneous, non-major histocompatibility complex restricted cytotoxic activity. NCC and LAK lyse a variety of transformed murine and human B-cell, T-cell and myeloid targets. A 32 kDa membrane protein [non-specific cytotoxic cell receptor protein (NCCRP-1)] expressed by NCC and certain mammalian NK/LAK cells mediates this cytotoxicity. NCCRP-1 is evolutionarily conserved and is found in species ranging from marine and freshwater teleosts to higher mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Natural killer cell frequency and function in Yorkshire pigs selectively bred for high or low antibody and cell-mediated immune response.
MA1-760 was used in flow cytometry to investigate the difference of NK cell function in pigs with different levels of immune response
|Raymond C,Wilkie B,Mallard B,Quinton M||Natural immunity (16:127)||2001|
Characterization of equine natural killer and IL-2 stimulated lymphokine activated killer cell populations.
MA1-760 was used in flow cytometry to investigate the characteristics of NK cells in horses
|Viveiros MM,Antczak DF||Developmental and comparative immunology (23:521)||1999|
Nonspecific cytotoxic cell receptor protein-1: a novel (predicted) type III membrane receptor on the teleost equivalent of natural killer cells recognizes conventional antigen.
MA1-760 was used in ELISA to determine the antigen binding region in nonspecific cytotoxic cell receptor protein 1
|Evans DL,Leary JH,Jaso-Friedmann L||Cellular immunology (187:19)||1998|
NCCRP-1: a novel receptor protein sequenced from teleost nonspecific cytotoxic cells.
MA1-760 was used in western blot to investigate the characteristics of NCCRP-1 in teleost fishes
|Jaso-Friedmann L,Leary JH,Evans DL||Molecular immunology (34:955)||1998|
Natural Killer Cell; NCC/NK