|Tested species reactivity||Yeast|
|Host / Isotype||Mouse / IgG3|
|Immunogen||Yeast nuclear preparation.|
|Storage buffer||tissue culture supernatant|
|Contains||5mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2-4 µl/lysate|
|Western Blot (WB)||1:10,000-1:25,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-10025 detects Yeast NOP-1 from S. cerevisiae samples and does not cross-react with fibrillarin in other species.
For chemiluminescent Western blot using yeast samples, use a dilution of 1:10,000 for cell lysates and 1:25,000 for nuclear fractions. For non-ECL detection methods, use a dilution of 1:1000-1:5000.
Nop1p is the yeast homologue of human fibrillarin, a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.