|Tested species reactivity||Not Applicable|
|Published species reactivity||Human|
|Host / Isotype||Goat / Ig Mix|
|Storage buffer||whole serum diluted in PBS, pH 7.6|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Blocking Assay (BLOCK)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.
Restoration and Storage: Store product at 4°C until opened. Restore with 10.0 ml distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, freeze rehydrated undiluted product in small aliquots at 20°C.
Reconstitute with 10.0 ml of distilled water.
Country of Origin: USA
Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates, making it useful for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients.
31873 was used in blocking or activating experiment to develop a validation protocol for sandwich elisa of serum samples from arthritic patients that eliminates data artifacts caused by anti-animal IgG antibodies
|Kragstrup TW,Vorup-Jensen T,Deleuran B,Hvid M||SpringerPlus (2:null)||2013|