|Tested species reactivity||Human|
|Host / Isotype||Rabbit / Ig Mix|
|Immunogen||Human serum, C4 free|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunodiffusion (IDF)||Assay Dependent|
|Immunoelectrophoresis (IE)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Titer results: Gel double diffusion titer : 1:>32. Gel double diffusion titer is defined as the dilution of antiserum sufficient to form a precipitate arc with 5ug antigen and 5ul per well.
Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species. Normal serum provides a sufficient quantity of endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum also supplies a native chemical environment for antibodies to function properly without adding foreign protein components. Normal serum is frequently used for blocking or saturating generalized binding interactions for immunodetection methods, especially those involving tissue samples such as immunohistochemistry (IHC). Normal sera are also useful as controls for testing general and specific antibody purification methods.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.