Western blot analysis of Notch 2 (cleaved) using Notch 2 (cleaved) polyclonal antibody (Product # PA5-37433) at a dilution of 1:500. Lane 1: HepG2 cell lysate treated with colchicine, Lane 2: Raw264.7 cell lysate treated with colchicine, Lane 3: Rat liver tissue lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 1734-1800 of human Notch 2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 85 and 110 kDa.
Purity is >95% by SDS-PAGE.
The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. To date, four Notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4. The Notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways. The Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged2 and Delta. Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.