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Immunohistochemical analysis of Npap60 + Nup50 in paraffin-embedded human uterus tissue using a Npap60 + Nup50 polyclonal antibody (Product # PA1-31827) at a dilution of 3.8 µg/ml. Steamed antigen retrieval was performed using a citrate buffer pH 6, followed by AP-staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence ELHKILLEKKDA, from C Terminus of the protein sequence according to NP_009103; NP_705931; NP_710151.|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31827 detects Npap60 + Nup50 from human samples.
PA1-31827 has been successfully used in Western blot applications.
The PA1-31827 immunogen is: Peptide with sequence ELHKILLEKKDA, from C Terminus of the protein sequence according to NP_009103; NP_705931; NP_710151.
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
50 kDa nucleoporin; CTA-268H5.7; MGC39961; NPAP60; NPAP60L; nuclear pore associated protein 60 kDa; Nuclear pore complex protein Nup50; Nuclear pore-associated protein 60 kDa-like; nuclear pore-associated protein 60L; nucleoporin 50kD; nucleoporin 50kDa; Nucleoporin Nup50; nucleoprotein 50
1700030K07Rik; AI413123; NPAP60; NPAP60L; NUP50; PRO1146