|Tested species reactivity||Yeast|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Yeast nuclear preparation.|
|Storage buffer||tissue culture supernatant|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:10,000-1:50,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-10029 detects Nsp1p from S. cerevisiae samples. To confirm a high affinity for Nsp1p, strain RS453, which expresses a shortened isoform of Nsp1p, was compared to the wildtype strain BJ5465. NSP1 in the RS453 strain contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for protein function, and encodes a protein that has been observed to migrate at ~85kDa on SDS-PAGE gels. Wildtype Nsp1p has a predicted MW of 86.5kDa but has been observed to migrate on SDS gels at ~100kDa. SDS-PAGE testing results showed wild type Nsp1p at ~108 kDa and Nsp1p RS453 at ~91kDa, confirming specificity for the Nsp1p protein.
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