Invitrogen

Nuclear Matrix Protein p84 Monoclonal Antibody (5E10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

1 Reference

View all (16) Nuclear Matrix Protein p84 antibodies

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Datasheet

Questions & Answers

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Nuclear Matrix Protein p84 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Nuclear Matrix Protein p84 was performed using 70% confluent log phase HCT 116 cells treated with 0.5 µg of Brefeldin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nuclear Matrix Protein p84 Mouse Monoclonal Antibody (Product # MA1-23261) at 5 microgram/ml in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e shows untreated cells with less Nuclear signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Nuclear Matrix Protein p84 Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of Nuclear Matrix Protein p84 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1 23261) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.

Nuclear Matrix Protein p84 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Nuclear Matrix Protein p84 was performed in paraffin-embedded human breast carcinoma tissue using Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Nuclear Matrix Protein p84 Antibody in Western Blot (WB) — Knockdown of Nuclear Matrix Protein p84 was achieved by transfecting HEK-293 cells with Nuclear Matrix Protein p84 specific siRNAs (Silencer® select Product # s19396; s19397). Western blot analysis (Fig. a) was performed using whole cell extracts from the Nuclear Matrix Protein p84 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Nuclear Matrix Protein p84.

Nuclear Matrix Protein p84 Antibody in Immunoprecipitation (IP) — MA1-23261 immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A: 30 µg whole cell lysate/extract of p84 protein expressing HepG2 cells B: Control with 3 µg of pre-immune mouse IgG C: Immunoprecipitation of p84 by 3 µg of p84 antibody (Product # MA1-23261) on 7.5% SDS-PAGE.

Nuclear Matrix Protein p84 Antibody in Cell treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Nuclear Matrix Protein p84 using Nuclear Matrix Protein p84 Mouse Monoclonal Antibody (Product # MA1-23261) shows increased expression of proteins in HCT 116 cell line upon Brefeldin treatment. {TM}

Nuclear Matrix Protein p84 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with Nuclear Matrix Protein p84 siRNA and reduction of signal was observed in Western Blot using Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261). {KD}

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

Assay Dependent

Immunocytochemistry (ICC)

0.5-2 µg/mL

Immunofluorescence (IF)

0.5-2 µg/mL

Immunohistochemistry (IHC)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

Assay Dependent

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

0.3-2 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Non-human primate, Rat

Published species

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5E10

Immunogen

Amino acids 15-374 of human p84 expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2202240

Product Specific Information

Suggested positive controls are HeLa, Raji, and MOLT4.

Target Information

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hTREX84; Nuclear matrix protein p84; p84N5; THO complex 1; THO complex subunit 1; Tho1

Gene Aliases: 3110002N20Rik; AW107452; HPR1; I79_014746; LRRGT00070; NMP-84; P84; P84N5; THOC1

UniProt ID: (Human) Q96FV9, (Mouse) Q8R3N6

Entrez Gene ID: (Human) 9984, (Chinese hamster) 100770844, (Mouse) 225160, (Rat) 291797

Molecular Function: nucleic acid binding

Function(s)

regulation of DNA recombination termination of RNA polymerase II transcription RNA processing RNA export from nucleus mRNA export from nucleus apoptotic process signal transduction RNA splicing mRNA 3'-end processing replication fork processing regulation of DNA-templated transcription, elongation positive regulation of DNA-templated transcription, elongation viral mRNA export from host cell nucleus negative regulation of isotype switching to IgA isotypes negative regulation of DNA damage checkpoint transcription, DNA-templated regulation of transcription, DNA-templated mRNA processing transport regulation of apoptotic process mRNA transport

Process(es)

DNA binding RNA binding protein binding

Nuclear Matrix Protein p84 Monoclonal Antibody (5E10)

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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