Invitrogen

Nuclear Matrix Protein p84 Monoclonal Antibody (5E10)

Advanced Verification

This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

View all (14) Nuclear Matrix Protein p84 antibodies

Datasheet

Datasheet

Product Image Gallery

Nuclear Matrix Protein p84 Antibody in Cell Treatment — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of Nuclear Matrix Protein p84 using Nuclear Matrix Protein p84 Mouse Monoclonal Antibody (Product # MA1-23261) shows increased expression of proteins in HCT 116 cell line upon Brefeldin treatment. {TM}

Nuclear Matrix Protein p84 Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with Nuclear Matrix Protein p84 siRNA and reduction of signal was observed in Western Blot using Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261). {KD}

Nuclear Matrix Protein p84 Antibody in Western Blot (WB) — Western blot analysis of p84 in Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction using a p84 monoclonal antibody (Product # MA1-23261).

Nuclear Matrix Protein p84 Antibody in Immunoprecipitation (IP) — MA1-23261 immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A: 30 µg whole cell lysate/extract of p84 protein expressing HepG2 cells B: Control with 3 µg of pre-immune mouse IgG C: Immunoprecipitation of p84 by 3 µg of p84 antibody (Product # MA1-23261) on 7.5% SDS-PAGE.

Nuclear Matrix Protein p84 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Nuclear Matrix Protein p84 was performed using 70% confluent log phase HCT 116 cells treated with 0.5 µg of Brefeldin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nuclear Matrix Protein p84 Mouse Monoclonal Antibody (Product # MA1-23261) at 5 microgram/ml in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e shows untreated cells with less Nuclear signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Product Details

Tested Applications

Dilution

ChIP assay (ChIP)

Assay Dependent

Immunocytochemistry (ICC)

5 µg/mL

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (IHC)

Assay Dependent

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5E10

Immunogen

Amino acids 15-374 of human p84 expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2202240

Product Specific Information

MA1-23261 detects p84 (MS - P8410 - PX1) in Human, mouse, rat and monkey samples. Suggested positive controls are HeLa, Raji, and MOLT4.

MA1-23261 has been successfully used in Immunofluorescence, Immunoprecipitation Immunohistochemistry, ChIP, and Western Blot procedures.

MA1-23261 immunogen corresponds to Amino acids 15-374 of human p84 expressed in E. coli..

Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.

Target Information

P84/N5-5E10 recognizes an 84 kDa nuclear matrix protein. Initially isolated by its interaction with the amino terminal region of the retinoblastoma protein, it has been used extensively as a nuclear-specific marker in both immunofluorescence and cell fractionation studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.