Invitrogen

Nuclear Matrix Protein p84 Monoclonal Antibody (5E10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Reference

View all (17) Nuclear Matrix Protein p84 antibodies

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Datasheet

Protocols

Questions & Answers

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Nuclear Matrix Protein p84 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Nuclear Matrix Protein p84 was performed using 70% confluent log phase HCT 116 cells treated with 0.5 µg of Brefeldin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Nuclear Matrix Protein p84 Mouse Monoclonal Antibody (Product # MA1-23261) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e shows untreated cells with less Nuclear signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Nuclear Matrix Protein p84 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of Nuclear Matrix Protein p84 was performed in paraffin-embedded human breast carcinoma tissue using Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261) at a dilution of 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Nuclear Matrix Protein p84 Antibody in Western Blot (WB) — Knockdown of Nuclear Matrix Protein p84 was achieved by transfecting HEK-293 cells with Nuclear Matrix Protein p84 specific siRNAs (Silencer® select Product # s19396; s19397). Western blot analysis (Fig. a) was performed using whole cell extracts from the Nuclear Matrix Protein p84 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Nuclear Matrix Protein p84.

Nuclear Matrix Protein p84 Antibody in Immunoprecipitation (IP) — MA1-23261 immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A: 30 µg whole cell lysate/extract of p84 protein expressing HepG2 cells B: Control with 3 µg of pre-immune mouse IgG C: Immunoprecipitation of p84 by 3 µg of p84 antibody (Product # MA1-23261) on 7.5% SDS-PAGE.

Nuclear Matrix Protein p84 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with Nuclear Matrix Protein p84 siRNA and reduction of signal was observed in Western Blot using Nuclear Matrix Protein p84 Monoclonal Antibody (5E10) (Product # MA1-23261). {KD}

Product Details

MA1-23261

Applications	Tested Dilution	Publications
Western Blot (WB)	0.3-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	Assay-dependent	-
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	0.5-2 µg/mL	-
Immunoprecipitation (IP)	1:100-1:500	-
ChIP assay (ChIP)	Assay-dependent	-

Product Specifications
Species Reactivity	Hamster, Human, Mouse, Non-human primate, Rat
Published species	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	5E10
Immunogen	Amino acids 15-374 of human p84 expressed in E. coli. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2202240

Product Specific Information

Suggested positive controls are HeLa, Raji, and MOLT4.

Target Information

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dihydroartemisinin inhibits the viability of cervical cancer cells by upregulating caveolin 1 and mitochondrial carrier homolog 2: Involvement of p53 activation and NAD(P)H:quinone oxidoreductase 1 downregulation.

International journal of molecular medicine

Zhang T,Hu Y,Wang T,Cai P

MA1-23261 was used in Western Blotting to identify Cav1 and MTCH2 as the molecular targets of DHA and revealed a new link between the upstream Cav1/MTCH2 upregulation and the downstream activation of the cell death pathway involved in the DHA-mediated inhibition of cell viability.