Western blot analysis of NRIP in human muscle tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse muscle tissue lysate and 4) rat muscle tissue lysate using a Nuclear Rec. Interacting Protein polyclonal antibody (Product # PA1-41255) at 1 ug/ml.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 800-850 of human NRIP.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: human skeletal muscle protein or tissue.
NRIP is a novel 860a.a nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up regulates their transcriptional activity, thereby functioning as a nuclear receptor coactivator. Role of NRIP has been implicated in cell growth and also in cervical and prostrate cancer thus indicating a therapeutic intervention. Northern Blot analysis detected a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.
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