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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 16 amino acid peptide near the center of human Nucleobindin-2|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||25 ug/ml|
|Western Blot (WB)||0.5 ug/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is rat brain tissue lysate.
PA5-34526 can be used with blocking peptide PEP-1569.
NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA-binding protein NEFA; epididymis secretory protein Li 109; Gastric cancer antigen Zg4; NEFA; nesfatin 1; nesfatin-1; NUCB2; Nucleobindin-2; nucleobinding 2; prepronefastin; Prepronesfatin
AI607786; Calnuc; HEL-S-109; NEFA; Nesfatin-1; NUCB2; p54