|Tested species reactivity||Dog, Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Nuclear matrix extract from human MDCK cells.|
|Storage buffer||tissue culture supernatant with 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-25778 detects Nucleolin from monkey, human, and canine samples. MA1-25778 will not react with mouse or rat.
MA1-25778 has been successfully used in immunoprecipitation, ELISA, immunocytochemistry and Western blot procedures. Suggested positive controls for this product are HeLa nuclear extract/HeLa cells.
The MA1-25778 immunogen is a nuclear matrix extract from human MDCK cells.
Store at 4°C short term. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.