Immunofluorescent analysis of Nucleophosmin (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Nucleophosmin monoclonal antibody (Product # MA5-12508) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG, kappa|
|Immunogen||GST fusion protein containing the N-terminal part of nucleophosmin fused to 14 aa of ALK protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12508 targets Nucleophosmin in IHC (P), ICC/IF and WB applications and shows reactivity with human and mouse samples.
The MA5-12508 immunogen is gST fusion protein containing the N-terminal part of nucleophosmin fused to 14 aa of ALK protein.
Nucleophosmin (NPM)/B23/numatrin is a phosphoprotein associated with cell nucleoli. Treatment of cells with cytotoxic drugs may make nucleophosmin enter nucleoplasm. It is reported that nucleophosmin shuttles continuously between cytoplasm and nucleoli to carry newly synthesized ribosomal proteins into nucleoli. NPM-ALK is a hybrid of the anaplastic lymphoma kinase (ALK) gene and the nucleophosmin (NPM) gene resulting from the t(2;5)(p23;q35) translocation found in anaplastic large cell lymphoma. In acute promyelocytic leukemia NPM gene is fused to RARalpha gene in (5;17) (q33;q12) transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deregulated expression of Nucleophosmin 1 in gastric cancer and its clinicopathological implications.
MA5-12508 was used in immunohistochemistry (paraffin) to examine NPM1 gene and protein expression in gastric tumors.
|Leal MF,Mazzotti TK,Calcagno DQ,Cirilo PD,Martinez MC,Demachki S,Assumpção PP,Chammas R,Burbano RR,Smith MC||BMC gastroenterology (14:null)||2014|
Oct4/Sox2 binding sites contribute to maintaining hypomethylation of the maternal igf2/h19 imprinting control region.
MA5-12508 was used in western blot to study maternal Igf2/H19 imprinting control region hypomethylation and the role of binding sites for Oct4 and Sox2
|Zimmerman DL,Boddy CS,Schoenherr CS||PloS one (8:null)||2013|
Functional characterization of the kinase activation loop in nucleophosmin (NPM)-anaplastic lymphoma kinase (ALK) using tandem affinity purification and liquid chromatography-mass spectrometry.
MA5-12508 was used in western blot to investigate the mechanism of KAL phosphorylation in NPM-ALK
|Wang P,Wu F,Ma Y,Li L,Lai R,Young LC||The Journal of biological chemistry (285:95)||2010|
Sindbis virus usurps the cellular HuR protein to stabilize its transcripts and promote productive infections in mammalian and mosquito cells.
MA5-12508 was used in immunocytochemistry to investigate the mechanism through which Sindbis virus evades from the cellular RNA decay machinery
|Sokoloski KJ,Dickson AM,Chaskey EL,Garneau NL,Wilusz CJ,Wilusz J||Cell host and microbe (8:196)||2010|
Nucleophosmin, p53, and Ki-67 expression patterns on an oral squamous cell carcinoma tissue microarray.
MA5-12508 was used in immunohistochemistry to investigate the expression of nucleophosmin, p53, and Ki-67 in tissues from oral squamous cell carcinoma
|Coutinho-Camillo CM,Lourenço SV,Nishimoto IN,Kowalski LP,Soares FA||Human pathology (41:1079)||2010|