Immunofluorescent analysis of Nup358 (green) in HeLa cells. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 1% Blocker BSA in PBS (Product # 37525), each for 15 minutes at room temperature. Cells were stained with a Nup358 monoclonal antibody (Product # MA1-847) at a concentration of 10ug/ml in 1% Blocker BSA in PBS, followed by a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:1000 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI (Product # 46190). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant protein corresponding to 150 N-terminal residues of human Nup358.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:100 - 1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100 - 1:1000|
|Western Blot (WB)||1:100 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-847 detects Nup358/RanBP2 from human samples.
Nup358 is an essential protein needed for proper kinetochore function. Chromatin congregation and segregation are distributed in the absence of Nup358. The assembly of other kinetochore components is also strongly inhibited. Therefore, Nup358 plays an essential role in the association of kinetochore function and maturation and nuclear envelope breakdown.
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