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Western blot analysis of mouse cortical brain lysates using O-Linked N-Acetylglucosamine Monoclonal Antibody (MA1-072). Blots containing cortical extracts from 4 individual C57BL/6 mice (Lanes 1-4) were blocked with 5% milk in TBST, and probed with MA1-072 (1:1000), followed by a fluorophore-conjugated goat anti-mouse IgG secondary antibody. Data courtesy of the Innovators Program.
|Tested species reactivity||All|
|Published species reactivity||Rat, Fruit fly, Primate, Amphibian, Hamster, Fish, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Pore complex-lamina fraction purified from rat liver nuclear envelopes.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay Dependent|
|Dot blot (DB)||1/800|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 47 publications below|
|Immunoprecipitation (IP)||See 7 publications below|
|ChIP assay (ChIP)||See 2 publications below|
|Immunohistochemistry (IHC)||See 5 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|ELISA (ELISA)||See 1 publications below|
MA1-072 detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, virus and rat tissues.
MA1-072 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody recognizes up to eight different proteins from the NPC of approximately 210, 180, 145, 100, 63, 58, 54 and 45 kDa as well as other O-linked glycoproteins outside of the NPC. Immunofluorescence staining of the NPC with MA1-072 primarily labels NPC O-linked glycoproteins and has been successfully used on a wide variety of mammalian cells. Labeling occurs at the NPC, with most of the labeling at the cytoplasmic and/or nucleoplasmic margins, as well as within the nucleus.
The MA1-072 immunogen is pore complex-lamina fraction purified from rat liver nuclear envelopes.
Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC"e;s. NPC"e;s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Fruit fly||Not Cited||
O-GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster.
MA1-072 was used in western blot to investigate the role of O-GlcNAcylation in autophagy
|Park S,Lee Y,Pak JW,Kim H,Choi H,Kim JW,Roth J,Cho JW||Cellular and molecular life sciences : CMLS (72:3173)||2015|
|Not Applicable||Not Cited||
Elevated O-GlcNAcylation promotes colonic inflammation and tumorigenesis by modulating NF-κB signaling.
MA1-072 was used in western blot to demonstrate that O-GlcNAcylation contributes to colitis by altering NF-kappaB signaling.
|Yang YR,Kim DH,Seo YK,Park D,Jang HJ,Choi SY,Lee YH,Lee GH,Nakajima K,Taniguchi N,Kim JM,Choi EJ,Moon HY,Kim IS,Choi JH,Lee H,Ryu SH,Cocco L,Suh PG||Oncotarget (6:12529)||2015|
Exhausting treadmill running causes dephosphorylation of sMLC2 and reduced level of myofilament MLCK2 in slow twitch rat soleus muscle.
MA1-072 was used in western blot to test if exhausting in vivo treadmill running induces dephosphorylation, and if there are reciprocal changes in O-GlcNAcylation, of MLC2
|Hortemo KH,Aronsen JM,Lunde IG,Sjaastad I,Lunde PK,Sejersted OM||Physiological reports (3:null)||2015|
Inhibition of mTOR affects protein stability of OGT.
MA1-072 was used in western blot to study the decrease in protein O-GlcNAcylation and OGT following inhibition of mTOR catalytic activity
|Park S,Pak J,Jang I,Cho JW||Biochemical and biophysical research communications (453:208)||2014|
Increased O-GlcNAcylation reduces pathological tau without affecting its normal phosphorylation in a mouse model of tauopathy.
MA1-072 was used in western blot to study the mechanism by which O-GlcNAcylation prevents pathological agregation of tau in a murine tauopathy model
|Graham DL,Gray AJ,Joyce JA,Yu D,O'Moore J,Carlson GA,Shearman MS,Dellovade TL,Hering H||Neuropharmacology (79:307)||2014|
Potential regulation of human muscle plasticity by MLC2 post-translational modifications during bed rest and countermeasures.
MA1-072 was used in western blot to study the post-translation modification of soleus MLC2 during 60 days of bed rest and the effects of exercise and nutritional countermeasures
|Stevens L,Bastide B,Hedou J,Cieniewski-Bernard C,Montel V,Cochon L,Dupont E,Mounier Y||Archives of biochemistry and biophysics (540:125)||2013|
Generation and characterization of a rabbit monoclonal antibody site-specific for tau O-GlcNAcylated at serine 400.
MA1-072 was used in western blot to study a site-specific rabbit monoclonal antibody that recognozes serine 400 O-GlcNAcylated tau
|Cameron A,Giacomozzi B,Joyce J,Gray A,Graham D,Ousson S,Neny M,Beher D,Carlson G,O'Moore J,Shearman M,Hering H||FEBS letters (587:3722)||2013|
A chemical glycoproteomics platform reveals O-GlcNAcylation of mitochondrial voltage-dependent anion channel 2.
MA1-072 was used in western blot to study mitochondrial VDAC-2 O-GlcNAcylation using a glycoproteomics approach
|Palaniappan KK,Hangauer MJ,Smith TJ,Smart BP,Pitcher AA,Cheng EH,Bertozzi CR,Boyce M||Cell reports (5:546)||2013|
CREB regulates the expression of neuronal glucose transporter 3: a possible mechanism related to impaired brain glucose uptake in Alzheimer's disease.
MA1-072 was used in western blot to study the regulation of neuronal Glut3 expression by CREB and the significance for the lowered Glut3 expression and glucose uptake observed in Alzheimer's disease brain
|Jin N,Qian W,Yin X,Zhang L,Iqbal K,Grundke-Iqbal I,Gong CX,Liu F||Nucleic acids research (41:3240)||2013|
The transcription factor Sp1 is responsible for aging-dependent altered nucleocytoplasmic trafficking.
MA1-072 was used in western blot to study the role of the Sp1 transcription factor as a key regulator of nucleocytoplasmic trafficking-related genes
|Kim SY,Kang HT,Han JA,Park SC||Aging cell (11:1102)||2012|
The increase in O-linked N-acetylglucosamine protein modification stimulates chondrogenic differentiation both in vitro and in vivo.
MA1-072 was used in western blot to study the role of O-linked N-acetylglucosamine protein modification in insulin-induced chondrocyte differentiation
|Andrés-Bergós J,Tardio L,Larranaga-Vera A,Gómez R,Herrero-Beaumont G,Largo R||The Journal of biological chemistry (287:33615)||2012|
Modulation of dynamin-related protein 1 (DRP1) function by increased O-linked-β-N-acetylglucosamine modification (O-GlcNAc) in cardiac myocytes.
MA1-072 was used in western blot to study the role of O-linked-beta-N-acetylglucosamine modification in regulating DRP1 functionality in cardiac muscle cells
|Gawlowski T,Suarez J,Scott B,Torres-Gonzalez M,Wang H,Schwappacher R,Han X,Yates JR,Hoshijima M,Dillmann W||The Journal of biological chemistry (287:30024)||2012|
Rescue of the transcription factors Sp1 and NFI in human skin keratinocytes through a feeder-layer-dependent suppression of the proteasome activity.
MA1-072 was used in western blot to study the role of a feeder layer in suppressing proteasome degradation of Sp1 and NF1 transcription factors in human skin keratinocytes
|Duval C,Gaudreault M,Vigneault F,Touzel-Deschênes L,Rochette PJ,Masson-Gadais B,Germain L,Guérin SL||Journal of molecular biology (418:281)||2012|
O-GlcNAc signaling is essential for NFAT-mediated transcriptional reprogramming during cardiomyocyte hypertrophy.
MA1-072 was used in western blot to study the role of O-GlcNAc signaling in NFAT-mediated transcriptional reprogramming during cardiomyocyte hypertrophy
|Facundo HT,Brainard RE,Watson LJ,Ngoh GA,Hamid T,Prabhu SD,Jones SP||American journal of physiology. Heart and circulatory physiology (302:H2122)||2012|
A role for O-GlcNAcylation in setting circadian clock speed.
MA1-072 was used in western blot to study the setting of the Drosophila circadian clock pace and the role played by O-GlcNAcylation
|Kim EY,Jeong EH,Park S,Jeong HJ,Edery I,Cho JW||Genes & development (26:490)||2012|
Altered calcium signaling in colonic smooth muscle of type 1 diabetic mice.
MA1-072 was used in western blot to investigate the changes of calcium levels in colonic smooth muscle of type 1 diabetic mice
|Touw K,Chakraborty S,Zhang W,Obukhov AG,Tune JD,Gunst SJ,Herring BP||American journal of physiology. Gastrointestinal and liver physiology (302:G66)||2012|
Glucosamine-supplementation promotes endoplasmic reticulum stress, hepatic steatosis and accelerated atherogenesis in apoE-/- mice.
MA1-072 was used in western blot to investigate the effect of glucosamine-supplementation on endoplasmic reticulum, hepatic steatosis and atherogenesis in apoE-/- mice
|Beriault DR,Sharma S,Shi Y,Khan MI,Werstuck GH||Atherosclerosis (219:134)||2011|
Protein O-GlcNAcylation regulates Drosophila growth through the insulin signaling pathway.
MA1-072 was used in western blot to study the effect of orotein O-GlcNAcylation on Drosophila growth and its mechanism
|Park S,Park SH,Baek JY,Jy YJ,Kim KS,Roth J,Cho JW,Choe KM||Cellular and molecular life sciences : CMLS (68:3377)||2011|
O-linked-N-acetylglucosamine cycling and insulin signaling are required for the glucose stress response in Caenorhabditis elegans.
MA1-072 was used in western blot to investigate the involvement of insulin signaling and O-GlcNAcylation in glucose stress response in C. elegans
|Mondoux MA,Love DC,Ghosh SK,Fukushige T,Bond M,Weerasinghe GR,Hanover JA,Krause MW||Genetics (188:369)||2011|
Characterization of O-GlcNAcylation in starfish (Asterina pectinifera) development from fertilization to bipinnaria larva.
MA1-072 was used in western blot to investigate the role of protein O-GlcNAcylation in Starfish development
|Ogawa M,Adachi T,Ikegami S,Kato KH,Yamamoto A,Kamemura K||Bioscience, biotechnology, and biochemistry (75:358)||2011|
O-GlcNAcylation determines the solubility, filament organization, and stability of keratins 8 and 18.
MA1-072 was used in western blot to investigate the effect of O-GlcNAcylation on keratins 8 and 18
|Srikanth B,Vaidya MM,Kalraiya RD||The Journal of biological chemistry (285:34062)||2010|
OGA inhibition by GlcNAc-selenazoline.
MA1-072 was used in western blot to investigate the inhibitory effect of GlcNAc-selenazoline on O-GlcNAcase
|Kim EJ,Love DC,Darout E,Abdo M,Rempel B,Withers SG,Rablen PR,Hanover JA,Knapp S||Bioorganic & medicinal chemistry (18:7058)||2010|
Regulation of insulin receptor substrate 1 (IRS-1)/AKT kinase-mediated insulin signaling by O-Linked beta-N-acetylglucosamine in 3T3-L1 adipocytes.
MA1-072 was used in western blot to investigate the effect of O-linked beta-N-acetylglucosamine on insulin signalling in 3T3-L1 adipocytes
|Whelan SA,Dias WB,Thiruneelakantapillai L,Lane MD,Hart GW||The Journal of biological chemistry (285:5204)||2010|
Influence of glucosamine on glomerular mesangial cell turnover: implications for hyperglycemia and hexosamine pathway flux.
MA1-072 was used in western blot to investigate the effect of glucosamine on glomerular mesangial cell proliferation and death and its mechanism
|James LR,Le C,Scholey JW||American journal of physiology. Endocrinology and metabolism (298:E210)||2010|
Nuclear receptor liver X receptor is O-GlcNAc-modified in response to glucose.
MA1-072 was used in western blot to investigate the modification of liver X receptor after glucose treatment
|Anthonisen EH,Berven L,Holm S,Nygård M,Nebb HI,Grønning-Wang LM||The Journal of biological chemistry (285:1607)||2010|
Urea-induced ROS generation causes insulin resistance in mice with chronic renal failure.
MA1-072 was used in western blot to investigate the effect of ROS on insulin resistance
|D'Apolito M,Du X,Zong H,Catucci A,Maiuri L,Trivisano T,Pettoello-Mantovani M,Campanozzi A,Raia V,Pessin JE,Brownlee M,Giardino I||The Journal of clinical investigation (120:203)||2010|
Dissecting the signaling events that impact classical nuclear import and target nuclear transport factors.
MA1-072 was used in western blot to investigate the effect of stress on nuclear trafficking
|Kodiha M,Tran D,Morogan A,Qian C,Stochaj U||PloS one (4:null)||2009|
Mapping of O-linked beta-N-acetylglucosamine modification sites in key contractile proteins of rat skeletal muscle.
MA1-072 was used in western blot to map the O-linked beta-N-acetylglucosamine modification sites in key contractile proteins of rat skeletal muscle
|Hédou J,Bastide B,Page A,Michalski JC,Morelle W||Proteomics (9:2139)||2009|
Galectin-4 is involved in p27-mediated activation of the myelin basic protein promoter.
MA1-072 was used in western blot to investigate the effect of galectin-4 on the p27-mediated activation of the MBP gene
|Wei Q,Eviatar-Ribak T,Miskimins WK,Miskimins R||Journal of neurochemistry (101:1214)||2007|
O-linked N-acetylglucosaminyltransferase inhibition prevents G2/M transition in Xenopus laevis oocytes.
MA1-072 was used in western blot to investigate the relationship between O-GlcNAc glycosylation and the regulation of G2/M transition in Xenopus oocytes
|Dehennaut V,Lefebvre T,Sellier C,Leroy Y,Gross B,Walker S,Cacan R,Michalski JC,Vilain JP,Bodart JF||The Journal of biological chemistry (282:12527)||2007|
Reduction of O-GlcNAc protein modification does not prevent insulin resistance in 3T3-L1 adipocytes.
MA1-072 was used in western blot to study the role of O-GlcNAc protein modification in insulin resistance.
|Robinson KA,Ball LE,Buse MG||American journal of physiology. Endocrinology and metabolism (292:E884)||2007|
Caenorhabditis elegans ortholog of a diabetes susceptibility locus: oga-1 (O-GlcNAcase) knockout impacts O-GlcNAc cycling, metabolism, and dauer.
MA1-072 was used in western blot to study the effect of O-GlcNAcase knockout in C. elegans.
|Forsythe ME,Love DC,Lazarus BD,Kim EJ,Prinz WA,Ashwell G,Krause MW,Hanover JA||Proceedings of the National Academy of Sciences of the United States of America (103:11952)||2006|
Stabilization of plakoglobin and enhanced keratinocyte cell-cell adhesion by intracellular O-glycosylation.
MA1-072 was used in western blot to study the effect of intracellular O-glycosylation on plakoglobin stabilization and keratinocyte cell-cell adhesion.
|Hu P,Berkowitz P,Madden VJ,Rubenstein DS||The Journal of biological chemistry (281:12786)||2006|
Why does acute hyperglycemia worsen the outcome of transient focal cerebral ischemia? Role of corticosteroids, inflammation, and protein O-glycosylation.
MA1-072 was used in western blot to study why acute hyperglycemia worsens the outcome of transient focal cerebral ischemia
|Martín A,Rojas S,Chamorro A,Falcón C,Bargalló N,Planas AM||Stroke; a journal of cerebral circulation (37:1288)||2006|
The feeder layer-mediated extended lifetime of cultured human skin keratinocytes is associated with altered levels of the transcription factors Sp1 and Sp3.
MA1-072 was used in western blot to study the role of Sp1 and Sp3 transcription factors in feeder layer-mediated extention of cultured keratinocyte lifespan
|Masson-Gadais B,Fugère C,Paquet C,Leclerc S,Lefort NR,Germain L,Guérin SL||Journal of cellular physiology (206:831)||2006|
Insulin dynamically regulates calmodulin gene expression by sequential o-glycosylation and phosphorylation of sp1 and its subcellular compartmentalization in liver cells.
MA1-072 was used in western blot to investigate the mechanism for insulin regulation of calmodulin gene expression in liver cells..
|Majumdar G,Harrington A,Hungerford J,Martinez-Hernandez A,Gerling IC,Raghow R,Solomon S||The Journal of biological chemistry (281:3642)||2006|
Posttranslational, reversible O-glycosylation is stimulated by high glucose and mediates plasminogen activator inhibitor-1 gene expression and Sp1 transcriptional activity in glomerular mesangial cells.
MA1-072 was used in western blot to investigate the effect of O-glycosylation on plasminogen activator inhibitor-1 gene expression and Sp1 transcriptional activity in glomerular mesangial cells..
|Goldberg HJ,Whiteside CI,Hart GW,Fantus IG||Endocrinology (147:222)||2006|
Nuclear localization of STAT5A modified with O-linked N-acetylglucosamine and early involution in the mammary gland of Hirosaki hairless rat.
MA1-072 was used in western blot to investigate the O-linked N-acetylglucosamine modification of STAT5A and its effect
|Nanashima N,Asano J,Hayakari M,Nakamura T,Nakano H,Yamada T,Shimizu T,Akita M,Fan Y,Tsuchida S||The Journal of biological chemistry (280:43010)||2005|
Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
MA1-072 was used in western blot to investigate the changes of O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1 during insulin stimulation and diabetes
|Majumdar G,Wright J,Markowitz P,Martinez-Hernandez A,Raghow R,Solomon SS||Diabetes (53:3184)||2004|
Identification of O-linked N-acetylglucosamine proteins in rat skeletal muscle using two-dimensional gel electrophoresis and mass spectrometry.
MA1-072 was used in western blot to suggest that O-GlcNAc modification could regulate skeletal muscle physiology
|Cieniewski-Bernard C,Bastide B,Lefebvre T,Lemoine J,Mounier Y,Michalski JC||Molecular & cellular proteomics : MCP (3:577)||2004|
|Not Applicable||Not Cited||
26S proteasome subunits are O-linked N-acetylglucosamine-modified in Drosophila melanogaster.
MA1-072 was used in western blot to study the glycosylation of 26S proteasome subunits in Drosophila melanogaster
|Sümegi M,Hunyadi-Gulyás E,Medzihradszky KF,Udvardy A||Biochemical and biophysical research communications (312:1284)||2003|
Down-regulation of Sp1 activity through modulation of O-glycosylation by treatment with a low glucose mimetic, 2-deoxyglucose.
MA1-072 was used in western blot to study the mechanism of down-regulation of SP1 activity by 2-DG.
|Kang HT,Ju JW,Cho JW,Hwang ES||The Journal of biological chemistry (278:51223)||2003|
High glucose and insulin promote O-GlcNAc modification of proteins, including alpha-tubulin.
MA1-072 was used in western blot to study the role of high glucose and insulin in the O-GlcNAc modification of proteins
|Walgren JL,Vincent TS,Schey KL,Buse MG||American journal of physiology. Endocrinology and metabolism (284:E424)||2003|
Enhanced O-GlcNAc protein modification is associated with insulin resistance in GLUT1-overexpressing muscles.
MA1-072 was used in western blot to investigate the role of the O-GlcNAc protein modification in insulin resistance
|Buse MG,Robinson KA,Marshall BA,Hresko RC,Mueckler MM||American journal of physiology. Endocrinology and metabolism (283:E241)||2002|
Flux through the hexosamine pathway is a determinant of nuclear factor kappaB- dependent promoter activation.
MA1-072 was used in western blot to study the effect of the increased flux through the hexosamine pathway on NF-kappaB-dependent promoter activation
|James LR,Tang D,Ingram A,Ly H,Thai K,Cai L,Scholey JW||Diabetes (51:1146)||2002|
Modulation of CCAAT/enhancer-binding protein-alpha gene expression by metabolic signals in rodent adipocytes.
MA1-072 was used in western blot to study the role of the metabolic signals during the modulation of CCAAT/enhancer-binding protein-alpha gene expression
|Wang Y,Lee-Kwon W,Martindale JL,Adams L,Heller P,Egan JM,Bernier M||Endocrinology (140:2938)||1999|
Interaction of the transcription factor Sp1 with the nuclear pore protein p62 requires the C-terminal domain of p62.
MA1-072 was used in western blot to investigate the interaction between Sp1 and p62
|Han I,Roos MD,Kudlow JE||Journal of cellular biochemistry (68:50)||1998|
Phospho-GlcNAc modulation of slow MLC2 during soleus atrophy through a multienzymatic and sarcomeric complex.
MA1-072 was used in immunoprecipitation to study the changes in slow isoform MLC2 phosphorylation and O-GlcNAcylation status during soleus muscle atrophy and the mechanisms involved
|Cieniewski-Bernard C,Dupont E,Richard E,Bastide B||Pflu¿gers Archiv : European journal of physiology (466:2139)||2014|
Requirement of decreased O-GlcNAc glycosylation of Mef2D for its recruitment to the myogenin promoter.
MA1-072 was used in immunoprecipitation to study the inverse relationship between Mef2D O-GlcNAc glycosylation and its recruitment to the myogenin promoter
|Ogawa M,Sakakibara Y,Kamemura K||Biochemical and biophysical research communications (433:558)||2013|
|Not Applicable||Not Cited||
O-GlcNAcylation increases ChREBP protein content and transcriptional activity in the liver.
MA1-072 was used in immunoprecipitation to study the regulation of carbohydrate-responsive element-binding protein (ChREBP) in the liver
|Guinez C,Filhoulaud G,Rayah-Benhamed F,Marmier S,Dubuquoy C,Dentin R,Moldes M,Burnol AF,Yang X,Lefebvre T,Girard J,Postic C||Diabetes (60:1399)||2011|
High glucose increases angiopoietin-2 transcription in microvascular endothelial cells through methylglyoxal modification of mSin3A.
MA1-072 was used in immunoprecipitation to investigate the role of high glucose in the increase of angiopoietin-2 transcription.
|Yao D,Taguchi T,Matsumura T,Pestell R,Edelstein D,Giardino I,Suske G,Rabbani N,Thornalley PJ,Sarthy VP,Hammes HP,Brownlee M||The Journal of biological chemistry (282:31038)||2007|
The tumor suppressor HIC1 (hypermethylated in cancer 1) is O-GlcNAc glycosylated.
MA1-072 was used in immunoprecipitation to investigate the O-GlcNAc modification of HIC1 (hypermethylated in cancer 1)
|Lefebvre T,Pinte S,Guérardel C,Deltour S,Martin-Soudant N,Slomianny MC,Michalski JC,Leprince D||European journal of biochemistry / FEBS (271:3843)||2004|
Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis.
MA1-072 was used in immunoprecipitation to study the role of cytosolic O-glycosylation of E-cadherin in blocking its transport to the cell surface during apoptosis
|Zhu W,Leber B,Andrews DW||The EMBO journal (20:5999)||2001|
The potential mechanism of the diabetogenic action of streptozotocin: inhibition of pancreatic beta-cell O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase.
MA1-072 was used in immunoprecipitation to study the inhibition of O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase as the potential diabetogenic mechanism of streptozotocin
|Konrad RJ,Mikolaenko I,Tolar JF,Liu K,Kudlow JE||The Biochemical journal (356:31)||2001|
Epigenetic regulation of a brain-specific glycosyltransferase N-acetylglucosaminyltransferase-IX (GnT-IX) by specific chromatin modifiers.
MA1-072 was used in ChIP assay and western blot to study the epigenetic regulatory mechanisms underlying the brain-specific expression of N-acetylglucosaminyltransferase-IX
|Kizuka Y,Kitazume S,Okahara K,Villagra A,Sotomayor EM,Taniguchi N||The Journal of biological chemistry (289:11253)||2014|
|Not Applicable||Not Cited||
Stress-induced expression of the p75 neurotrophin receptor is regulated by O-GlcNAcylation of the Sp1 transcription factor.
MA1-072 was used in ChIP assay to investigate the role of transcription factor Sp1 glycosylation during stress-induced expression of p75 neurotrophin receptor
|Kommaddi RP,Dickson KM,Barker PA||Journal of neurochemistry (116:396)||2011|
Increasing brain protein O-GlcNAc-ylation mitigates breathing defects and mortality of Tau.P301L mice.
MA1-072 was used in immunohistochemistry and western blot to study the beneficial effects of increasing the O-GlucNAcylation of brain proteins on the survival and breathing of aged tau P301L transgenic mice
|Borghgraef P,Menuet C,Theunis C,Louis JV,Devijver H,Maurin H,Smet-Nocca C,Lippens G,Hilaire G,Gijsen H,Moechars D,Van Leuven F||PloS one (8:null)||2013|
O-GlcNAcylation is a novel regulator of lung and colon cancer malignancy.
MA1-072 was used in immunohistochemistry to investigate the O-GlcNAcylation level in the lung and colon tumor tissues
|Mi W,Gu Y,Han C,Liu H,Fan Q,Zhang X,Cong Q,Yu W||Biochimica et biophysica acta (1812:514)||2011|
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
MA1-072 was used in immunohistochemistry to study the role of the myosin16b COOH-tail region in localization to the nucleus and S-phase progression
|Cameron RS,Liu C,Mixon AS,Pihkala JP,Rahn RJ,Cameron PL||Cell motility and the cytoskeleton (64:19)||2007|
Elevated expression of O-GlcNAc-modified proteins and O-GlcNAc transferase in corneas of diabetic Goto-Kakizaki rats.
MA1-072 was used in immunohistochemistry to elucidate the role of O-GlcNAc modification in the pathogenesis of diabetic keratopathy.
|Akimoto Y,Kawakami H,Yamamoto K,Munetomo E,Hida T,Hirano H||Investigative ophthalmology & visual science (44:3802)||2003|
Insulin-dependent activation of endothelial nitric oxide synthase is impaired by O-linked glycosylation modification of signaling proteins in human coronary endothelial cells.
MA1-072 was used in immunohistochemistry to investigate the role of O-linked glycosylation modification of signaling proteins in insulin-dependent activation of endothelial nitric oxide synthase
|Federici M,Menghini R,Mauriello A,Hribal ML,Ferrelli F,Lauro D,Sbraccia P,Spagnoli LG,Sesti G,Lauro R||Circulation (106:466)||2002|
Hexosamine biosynthesis is a possible mechanism underlying hypoxia's effects on lipid metabolism in human adipocytes.
MA1-072 was used in immunocytochemistry and western blot to study the role of hexosamine biosynthesis in the mechanism by which hypoxia modulates human adipocyte lipid metabolism
|O'Rourke RW,Meyer KA,Gaston G,White AE,Lumeng CN,Marks DL||PloS one (8:null)||2013|
Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.
MA1-072 was used in immunocytochemistry and immunohistochemistry to study the role of the interaction of surface vimentin with O-GlcNAc-modified proteins in the engulfment clearance of apoptotic cells
|Ise H,Goto M,Komura K,Akaike T||Glycobiology (22:788)||2012|
Fat-induced membrane cholesterol accrual provokes cortical filamentous actin destabilisation and glucose transport dysfunction in skeletal muscle.
MA1-072 was used in immunocytochemistry to investigate the destabilisation of cortical filamentous actin and dysfunction of glucose transport induced by accrual
|Habegger KM,Penque BA,Sealls W,Tackett L,Bell LN,Blue EK,Gallagher PJ,Sturek M,Alloosh MA,Steinberg HO,Considine RV,Elmendorf JS||Diabetologia (55:457)||2012|
Immunochemical methods for the rapid screening of the o-glycosidically linked N-acetylglucosamine modification of proteins.
MA1-072 was used in ELISA to evaluate the efficacy and applications of two monoclonal antibodies against o-glycosidically linked N-acetylglucosamine modification of proteins
|Ahrend M,Käberich A,Fergen MT,Schmitz B||Methods in molecular biology (Clifton, N.J.) (446:267)||2008|
GlcNAc; O-GlcNAc transferase p110 subunit; O-GlcNAc transferase subunit p110; O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase); O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase; uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
HINCUT-1; HRNT1; O-GLCNAC; OGT